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    His-tag ubiquitin activating Enzyme (His-UBE1)

    產品編號 規格 價格 貨期
    UBE-024 25ug ¥1280 現貨
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    Description
        This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its C-terminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. UBE1 is a critical compo-nent for the initiation of any in vitro conjugation reactions. Protein has tag at the N-terminus. 
    使用我公司的UBE1能夠將泛素催化形成泛素鏈(如下圖)。

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